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KMID : 0604620060130020064
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Dongguk Journal of Medicine
2006 Volume.13 No. 2 p.64 ~ p.72
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Apoptotic Effect of Hedyotis Diffusa in Gastric Cancer Cell Lines
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Park Jeong-Bae
Jang Suk-Yong
Lee Jong-Im
Park Keon-Uk
Sung Nak-Il
Lee Joon-Yeop
Hur In-Gyung
Kim Seung-Min
Nam Ji-Hyung
Lee Yong-Kook
Choi Jae-Hyeuk
Baek Jin-Ho
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Abstract
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Hedyotis diffusa has been used for cancer treatment in Korea and China. For the evaluation of anti-tumor activity through apoptosis, our experiments were performed with methanol extracts of Hedyotis diffusa on gastric cancer cell lines such as SNU-719, 601, 668. The cells were treated with various concentrations and periods of methanol extracts. Then, the cell viability was measured by trypan blue dye exclusion test. For the evaluation of apoptosis, we calculated the population of subG1 cells by the flow cytometry method, and examined the expression of caspase-3 by Western blot analysis. In SNU-719 and 601 cell lines, the anti-tumor activity of methanol extracts were not shown. However in SNU-668 cell lines, the anti-tumor activity was detected. The cell viability of SNU-668 was significantly decreased in a dose-dependent manner at 200 and 400 ㎍/ml of methanol extracts (0 ㎍/ml : 94.3±0.9%, 1 ㎍/ml : 95.8±0.8%, 5 ㎍/ml : 93.5±1.6%, 25 ㎍/ml : 95.3±2.0%, 50 ㎍/ml : 93.3±2.5%, 100 ㎍/ml : 93.5±3.8%, 200 ㎍/ml : 83.9±4.3%, 400 ㎍/ml : 72.8±8.4%). The p-values were 0.013 and 0.005 in between 100 ㎍/ml and 200 ㎍/ml, and 200 ㎍/ml and 400 ㎍/ml, respectively. The cell viability of SNU-668 was significantly decreased in a time-dependent manner as well (0 hr. : 98.8±1.1%, 6 hr. 83.2±8.6%, 24 hr. : 37.6±8.3%, 48 hr. : 22.2±11.2%, p=0.019). In the flow cytometry, the number of cells of subG1 population were increased in both dose-and time-dependent manners. In the Western blot analysis, caspase-3 proenzyme levels were decreased in both dose- and time-dependent manners as well. In conclusion, this study shows that methanol extracts of Hedyotis diffusa are effective on the apoptosis of SNU-668 gastric cancer cells. Therefore, through the analysis of components and the study of apoptotic signaling pathways of methanol extracts of Hedyotis diffusa, we could make a new drugs for the treatment of gastric cancer.
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KEYWORD
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hedyotis, apoptosis, gastric cancer
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